Effects of mesoporous SiO2-CaO nanospheres on the murine peritoneal macrophages/Candidaalbicans interface

The use of nanoparticles for intracellular drug delivery could reduce the toxicity and side effects but, uptake these nanocarriers induce adverse on cells tissues after their incorporation. Macrophages play a central role in host defense are responsible vivo nanoparticle trafficking. Assessment capacity against pathogenic micro-organisms uptake, is necessary to prevent infections associated with therapies. In this study, hollow mesoporous SiO2-CaO nanospheres labeled fluorescein isothiocyanate (FITC-NanoMBGs) function peritoneal macrophages was assessed by measuring ability phagocytize Candida albicans expressing red fluorescent protein. Two macrophage/fungus ratios (MOI 1 MOI 5) were used two experimental strategies carried out: a) pretreatment FITC-NanoMBGs subsequent fungal infection; b) competition assays simultaneous addition fungus nanospheres. Macrophage pro-inflammatory phenotype markers (CD80 expression interleukin 6 secretion) also evaluated. Significant decreases CD80+ macrophage percentage secretion observed 30 min, indicating that incorporation NanoMBG favors non-inflammatory phenotype. present study evidences all studied conditions does not alter function. Moreover, transitory increase phagocytosis at short time interaction. assays, as quantity increased, FITC-NanoMBG content decreased MOI- time-dependent manner. These results have confirmed clearly distinguish between inert material live yeast dynamic Furthermore, critical determinant know functional state valuable parameter nanomaterial / interface.